LOCUS       40924_39053        4439 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSDU1 deltamobdeltaorfEF, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Wang R, Lin C, Lin J, Pang X, Liu X, Zhang C, Lin J, Chen L.
  TITLE     Construction of novel pJRD215-derived plasmids using chloramphenicol
            acetyltransferase (cat) gene as a selection marker for 
            Acidithiobacillus caldus
  JOURNAL   PLoS ONE 12 (8), e0183307 (2017)
  PUBMED    28813510
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Wang R, Chen L, Lin J, Lin J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2017) State Key Laboratory of Microbial 
            Technology, School of Life Sciences, Shandong University, 27 Shanda 
            South Road, Jinan, Shandong 250100, China
REFERENCE   3  (bases 1 to 4439)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4439)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2017"; volume: "12"; issue: "8"; pages: "e0183307"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAY-2017) State Key Laboratory of Microbial Technology, School 
            of Life Sciences, Shandong University, 27 Shanda South Road, Jinan, 
            Shandong 250100, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: BioEdit v. 7.1.3.0
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(131..525)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             658..1626
                     /codon_start=1
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             1690..2526
                     /codon_start=1
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             2516..3364
                     /codon_start=1
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /translation="VVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     misc_feature    complement(3561..3617)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     CDS             3706..4362
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gtgcactcct tgcaatactg tgtttacata cagtctatcg cttagcggaa agttctttta
       61 ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt cactcccgta
      121 ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact gtcacgaacc
      181 cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg gctggcgggg
      241 tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg ttatcagcgc
      301 ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga tagctggtct
      361 attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa aggcgggcaa
      421 agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt tagccgcttt
      481 agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca gggcctgtct
      541 cggtcgatca ttcagctccc cttaaccatc ttgacacccc attgttaatg tgctgtctcg
      601 taggctatca tggaggcaca gcattgagcc gaaaagcaaa agcaacagcg aggcagcatg
      661 aagaacgaca ggactttgca ggccataggc cgacagctca aggccatggg ctgtgagcgc
      721 ttcgatatcg gcgtcaggga cgccaccacc ggccagatga tgaaccggga atggtcagcc
      781 gccgaagtgc tccagaacac gccatggctc aagcggatga atgcccaggg caatgacgtg
      841 tatatcaggc ccgccgagca ggagcggcat ggtctggtgc tggtggacga cctcagcgag
      901 tttgacctgg atgacatgaa agccgagggc cgggagcctg ccctggtagt ggaaaccagc
      961 ccgaagaact atcaggcatg ggtcaaggtg gccgacgccg caggcggtga acttcggggg
     1021 cagattgccc ggacgctggc cagcgagtac gacgccgacc cggccagcgc cgacagccgc
     1081 cactatggcc gcttggcggg cttcaccaac cgcaaggaca agcacaccac ccgcgccggt
     1141 tatcagccgt gggtgctgct gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg
     1201 ctggtgcagc aggctggcca gcagatcgag caggcccagc ggcagcagga gaaggcccgc
     1261 aggctggcca gcctcgaact gcccgagcgg cagcttagcc gccaccggcg cacggcgctg
     1321 gacgagtacc gcagcgagat ggccgggctg gtcaagcgct tcggtgatga cctcagcaag
     1381 tgcgacttta tcgccgcgca gaagctggcc agccggggcc gcagtgccga ggaaatcggc
     1441 aaggccatgg ccgaggccag cccagcgctg gcagagcgca agcccggcca cgaagcggat
     1501 tacatcgagc gcaccgtcag caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc
     1561 gagctggcac gggcaccggc accccgccag cgaggcatgg acaggggcgg gccagatttc
     1621 agcatgtagt gcttgcgttg gtactcacgc ctgttatact atgagtactc acgcacagaa
     1681 gggggtttta tggctaccca taagcctatc aatattctgg aggcgttcgc agcagcgccg
     1741 ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc gctggtgtcg
     1801 cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat tgcaggcggg
     1861 ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct gcccgccgaa
     1921 gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct cagcgccgag
     1981 gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag cctgcccaac
     2041 atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg ccgcctgatg
     2101 gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg ccccatggcc
     2161 caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat cgtgttcctg
     2221 caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca ggccagccgg
     2281 ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag catgaccagc
     2341 gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt ccgcttcggt
     2401 gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg gcatgacggc
     2461 ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt gccccgtggt
     2521 gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc actgtctggc
     2581 ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc tggacgtgac
     2641 gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc tgggcgctga
     2701 tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc tagtgcttgg
     2761 cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac ccaagtggga
     2821 ggccgtcacc gctgaatgcc atgtggtcaa aggtagctat cgggcgctgg caaaggaaat
     2881 cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca tcgagcgcct
     2941 ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc ggctgctgtc
     3001 ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc ccttgatcgc
     3061 gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg tgcgggcgct
     3121 ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg accccggcaa
     3181 aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag aggccagtgg
     3241 ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc tggtcgcgct
     3301 gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc ccaaggcggc
     3361 aggctgaccc cccccactct attgtaaaca agacattttt atcttttata ttcaatggct
     3421 tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag gcttaacaat
     3481 attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct ttcctggctt
     3541 tgcttccaga tgtatgctct aagcttgcat gcctgcaggt cgactctaga ggatccccgg
     3601 gtaccgagct cgaattcatg cattcatgtt tgacagctta tcatccataa ggtttaatgc
     3661 ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgga gaaaaaaatc
     3721 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     3781 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     3841 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
     3901 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
     3961 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
     4021 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
     4081 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
     4141 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
     4201 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
     4261 ccgctggcga ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt
     4321 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     4381 tattggtgcc cttaaacgcc tggtgctacg cctgaataag tgataataag cggatgaat
//
